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JoVE Journal Genetics
Flow-sortering og Exome Sequencing av Reed-Sternberg-cellene av klassisk Hodgkin-lymfom
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Flow-sortering og Exome Sequencing av Reed-Sternberg-cellene av klassisk Hodgkin-lymfom

Article DOI: 10.3791/54399-v • 08:53 min • June 10th, 2017
June 10th, 2017 •
1, 2, 3, 4, 5, 6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

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Her beskriver vi en kombinert flyt-cytometrisk celle sortering og lavinngang, neste generasjons bibliotekskonstruksjonsprotokoll utviklet for å produsere høykvalitets, hel-eksome data fra Hodgkin Reed-Sternberg (HRS) celler av klassisk Hodgkin lymfom (CHL).

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Genetikk utgave 124 klassisk Hodgkin lymfom cHL Reed-Sternberg FACS exome neste generasjons sekvenseringsbibliotek flytcellesortering
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