Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / CUBIC Protocol Visualizes Protein Expression at…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
Mounting the Mouse on the Live Imaging Insert for Intravital Imaging
Journal (Biology)
Mounting the Mouse on the Live Imaging Insert for Intravital Imaging
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
Journal (Biology)
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
 

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

Article DOI: 10.3791/54401-v • 08:09 min • August 4th, 2016
August 4th, 2016 •
*1,2, *1, *1, 3, 1, 1
1The School of Medical Sciences, University of New South Wales Australia, 2Neuroscience Research Australia, 3Biomedical Imaging Facility, University of New South Wales Australia
* These authors contributed equally

Chapters

Summary
Automatic Translation

This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.

Tags

CUBIC Protocol Protein Expression Single Cell Resolution Whole Mount Skin Preparations Visualize Cell Proliferation Three Dimensions Anatomy Pathology Epidermal Development Regeneration Skin Disease Technique Full Thickness Skin Biopsies Accuracy Epidermis Dermis Interactions Hair Follicles Betty Maclarios Ph.D. Student Developmental And Regenerative Dermatology Unit UNSW Australia Trimmers Shave Hair Decontaminate 70% Ethanol PBS Lift Dorsal Neck Skin Scissors
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter