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CUBIC Protocol Visualiseert Protein Expression op enkel cel resolutie in Whole Mount Skin Voorbereidingen
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CUBIC Protocol Visualiseert Protein Expression op enkel cel resolutie in Whole Mount Skin Voorbereidingen

Article DOI: 10.3791/54401-v • 08:09 min • August 4th, 2016
August 4th, 2016 •
*1,2, *1, *1, 3, 1, 1
1The School of Medical Sciences, University of New South Wales Australia, 2Neuroscience Research Australia, 3Biomedical Imaging Facility, University of New South Wales Australia
* These authors contributed equally

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Dit rapport beschrijft een KUBIEKE protocol om de volledige dikte muis huid biopten te verduidelijken en te visualiseren eiwit expressie patronen, delende cellen, en sebocyten bij de enkele resolutie cel in 3D. Deze methode maakt een nauwkeurige evaluatie van de huid anatomie en pathologie, en abnormale epidermale fenotypes in genetisch gemodificeerde muis lijnen.

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Cellular Biology huid epidermis CUBIC 3D imaging confocale muis
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