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JoVE Journal
Genetics

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河豚有毒改进的聚合酶链反应 - 限制性片段长度多态性分型液相色谱/质谱法
 
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河豚有毒改进的聚合酶链反应 - 限制性片段长度多态性分型液相色谱/质谱法

Article DOI: 10.3791/54402-v 09:34 min September 20th, 2016
September 20th, 2016

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Summary

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Click here for the English version.

描述了用于通过液相色谱/质谱基因型河豚物种的改进的聚合酶链式反应 - 限制性片段长度多态性方法。反相硅胶整料柱被用于分离消化的扩增子。这种方法可以阐明的寡核苷酸的单同位素质量,其是用于识别基础组合物是有用的。

Tags

遗传学,第115,生物化学,发行,DNA,寡核苷酸,单一同位素质量,河豚,碱基组成,电
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