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Journal / Cancer Research / Capture and Release van levensvatbare…
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Cancer Research

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JoVE Journal Cancer Research
Capture and Release van levensvatbare circulerende tumorcellen van Blood
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Capture and Release van levensvatbare circulerende tumorcellen van Blood

Article DOI: 10.3791/54435-v • 08:10 min • October 28th, 2016
October 28th, 2016 •
1, 2, 1,3
1Department of Pathology, University of Miami, 2SRI International, 3Department of Biomedical Engineering, DJTMF Biomedical Nanotechnology Institute at the University of Miami, University of Miami

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Een protocol om een poly (N--ISO propylacrylamide) (PIPAAm) gecoat microfilter voor een effectieve vangst en thermoresponsieve release van levensvatbare circulerende tumorcellen (CTC) wordt gepresenteerd te benutten. Deze methode maakt het vangen van CTC uit het bloed van de patiënt en de daaropvolgende vrijlating van levensvatbare CTC voor downstream off-chip cultuur, analyses en karakterisering.

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Cancer Research Circulerend Tumor Cell CTC cultuur PIPAAm Microfilter Live Cell Capture thermoresponsieve release Precision Medicine Personalized Medicine Translational Medicine Liquid biopsie
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