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Journal / Biochemistry / Single-molekyl Super-Resolution Imaging of…
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Biochemistry

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JoVE Journal Biochemistry
Single-molekyl Super-Resolution Imaging of phosphatidylinositol 4,5-bisfosfat i plasmamembranen med Novel fluorescerende prober
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Single-molekyl Super-Resolution Imaging of phosphatidylinositol 4,5-bisfosfat i plasmamembranen med Novel fluorescerende prober

Article DOI: 10.3791/54466-v • 07:26 min • October 15th, 2016
October 15th, 2016 •
1, 1
1Department of Neuroscience, University of Wisconsin-Madison

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PI (4,5) P 2 regulerer ulike cellulære funksjoner, men nanoskala organisasjon i cellen plasmamembranen er dårlig forstått. Ved merking PI (4,5) P-2 med en to-farge fluorescerende probe sammensmeltet til Pleckstrin Homologi domene, beskriver vi en ny tilnærming for å studere PI (4,5) P to romlig fordeling i plasmamembranen til nanometerskala .

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Biokjemi Super-Resolution Imaging PALM phosphatidylinositol 4,5-bisfosfat (PI (4,5) P2) cellemembranen ark phosphoinositide betaceller
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