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Journal / Developmental Biology / हीट शॉक 2 उपचार के साथ zebrafish भ्रूण की Ploidy…
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JoVE Journal Developmental Biology
हीट शॉक 2 उपचार के साथ zebrafish भ्रूण की Ploidy हेरफेर
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हीट शॉक 2 उपचार के साथ zebrafish भ्रूण की Ploidy हेरफेर

Article DOI: 10.3791/54492-v • 11:19 min • December 16th, 2016
December 16th, 2016 •
1, *1,2, *1,3, 1
1Laboratory of Genetics, University of Wisconsin, 2Department of Neurobiology, University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program, University of Pittsburgh School of Medicine
* These authors contributed equally

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ploidy हेरफेर के लिए एक संशोधित प्रोटोकॉल जल्दी भ्रूण में cytokinesis में एक एक चक्र स्टाल प्रेरित करने के लिए एक गर्मी झटका उपयोग करता है। इस प्रोटोकॉल zebrafish में प्रदर्शन किया है, लेकिन अन्य प्रजातियों के लिए लागू किया जा सकता है।

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विकास जीवविज्ञान अंक 118 zebrafish ploidy हेरफेर पूरे जीनोम दोहराव gynogenesis गर्मी झटका तारककेंद्रक दोहराव homozygous द्विगुणित
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