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Journal / Genetics / Genmanipulation av växtpatogen
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JoVE Journal Genetics
Genmanipulation av växtpatogen
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Genmanipulation av växtpatogen

Article DOI: 10.3791/54522-v • 11:42 min • September 30th, 2016
September 30th, 2016 •
*1,2, *1, 3, 3, 1,2, 1,2,4
1Institute for Microbiology, Heinrich-Heine University Düsseldorf, 2Bioeconomy Science Center (BioSC), 3Department of Genetics, Institute of Applied Biosciences, Karlsruhe Institute of Technology, 4Cluster of Excellence in Plant Sciences (CEPLAS), Heinrich-Heine University Düsseldorf
* These authors contributed equally

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Vi beskriver en robust gen ersättningsstrategi för att genetiskt manipulera smut svampen Ustilago maydis. Detta protokoll beskriver hur man skapar deletionsmutanter att undersöka infektion fenotyper. Den kan förlängas för att modifiera gener på något önskat sätt, t ex, genom att lägga till en sekvens som kodar för ett fluorescerande protein tagg.

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Genetik , Genteknik deletionsmutant omvänd genetik mutant fenotyp homolog rekombination modell smut svamp växt patogen stam kontroll
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