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Journal / Biochemistry / Rilevamento del pH-dipendente attività di
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Rilevamento del pH-dipendente attività di
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Rilevamento del pH-dipendente attività di

Article DOI: 10.3791/54527-v • 08:32 min • October 23rd, 2016
October 23rd, 2016 •
1, 1,2, 1,2, 1
1Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 2Howard Hughes Medical Institute, University of Michigan

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Questo studio descrive le tecniche biofisiche, biochimiche e molecolari per caratterizzare l'attività chaperone di Escherichia coli HdeB in condizioni di pH acido. Questi metodi sono stati applicati con successo per altri accompagnatori acido-protettivi come HdeA e può essere modificato a lavorare per altri accompagnatori e condizioni di stress.

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Biochimica chaperone molecolare stress acido risposta allo stress Proteina aggregazione proteine ​​Unfolding batteri Light Scattering Proteine ​​ripiegamento proteina-proteina interazione controllo di qualità delle proteine.
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