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Journal / Immunology and Infection / Infezione sperimentale con
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Immunology and Infection

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JoVE Journal Immunology and Infection
Infezione sperimentale con
Fecal (micro) RNA Isolation
Journal (Immunology and Infection)
Fecal (micro) RNA Isolation
Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
Journal (Immunology and Infection)
Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
 
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Infezione sperimentale con

Article DOI: 10.3791/54554-v • 10:10 min • November 16th, 2016
November 16th, 2016 •
*1, *1, *1, 1, 1,2,3
1Department of Immunology, University of Toronto, 2Toronto General Research Institute, University Health Network, 3Women's College Research Institute
* These authors contributed equally

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Questo protocollo descrive come inoculare topi C57BL / 6J con il ceppo EGD di Listeria monocytogenes (L. monocytogenes) e per misurare l'interferone-γ (IFN-γ) risposte da parte delle cellule natural killer (NK), cellule T natural killer (NKT), e adattiva linfociti T dopo l'infezione. Questo protocollo descrive anche come condurre studi di sopravvivenza in topi dopo l'infezione con una versione modificata del DL 50 dosi del patogeno.

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L'infezione Numero 117, Batteri modello di infezione i topi citometria a flusso la risposta interferon-γ
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