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Metoder för isolering, kultur, och funktionell karakterisering av sinusknutan Myocyter från vuxna möss
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Metoder för isolering, kultur, och funktionell karakterisering av sinusknutan Myocyter från vuxna möss

Article DOI: 10.3791/54555-v • 09:32 min • October 23rd, 2016
October 23rd, 2016 •
*1, *1,2, 1,3
1Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, 2Department of Bioengineering, University of Colorado Anschutz Medical Campus, 3Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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Metoder demonstreras för isolering av sinusknutan myocyter (SAMS) från vuxna möss för patch clamp elektrofysiologiska eller avbildning studier. Isolerade celler kan användas direkt eller kan hållas i odling för att tillåta uttryck av proteiner av intresse, såsom genetiskt kodade reportrar.

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Cellbiologi sinusknutan Pacemaking cellodling adenovirus elektrofysiologi rolig ström
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