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Metoder til isolering, Kultur og funktionel karakterisering af sinoatrialt Node Myocytter fra voksne mus
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Metoder til isolering, Kultur og funktionel karakterisering af sinoatrialt Node Myocytter fra voksne mus

Article DOI: 10.3791/54555-v • 09:32 min • October 23rd, 2016
October 23rd, 2016 •
*1, *1,2, 1,3
1Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, 2Department of Bioengineering, University of Colorado Anschutz Medical Campus, 3Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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Fremgangsmåder er påvist for isoleringen af ​​sinusknude myocytter (SAMS) fra voksne mus for patch clamp elektrofysiologi eller billeddannende undersøgelser. Isolerede celler kan anvendes direkte eller kan opretholdes i kultur for at tillade ekspression af proteiner af interesse, såsom genetisk indkodede reportere.

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Cellular Biology sinoatriale node pacemaking cellekultur adenovirus elektrofysiologi sjove strøm
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