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Journal / Immunology and Infection / Fænotypisk karakterisering af makrofager fra…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Fænotypisk karakterisering af makrofager fra rottenyre ved flowcytometri
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Fænotypisk karakterisering af makrofager fra rottenyre ved flowcytometri

Article DOI: 10.3791/54599-v • 07:14 min • October 18th, 2016
October 18th, 2016 •
1, 1, 2, 3, 2, 2, 3, 3, 3, 1, 1
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, 2Department of Physiology, Faculty of Medicine, Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)

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Dette håndskrift beskriver en detaljeret protokol for fænotypisk og kvantitativ analyse af hjemmehørende makrofager fra rotte nyrer ved flowcytometri. De resulterende farvede celler kan også bruges til andre formål, herunder celle sortering, genekspression analyse eller funktionelle studier, hvilket øger oplysninger indhentet i den eksperimentelle model.

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Immunologi makrofager Inflammation M1 M2 fænotyper polarisation Nyre Rat
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