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Journal / Biochemistry / A Method for Measuring RNA N6-methyladenosine…
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Biochemistry

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JoVE Journal Biochemistry
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Article DOI: 10.3791/54672-v • 08:56 min • December 5th, 2016
December 5th, 2016 •
1, 1, 1
1Department of Cardiology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine

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Summary
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A modified northern blotting method for measuring N6-methyladenosine (m6A) modifications in RNA is described. The current method can detect modifications in diverse RNAs and controls under various experimental designs.

Tags

RNA N6-methyladenosine Modifications Modified Northern Blotting Technique RNA Epigenetics N6A Modification Detection RNA Modification Detection Ribonuclease Decontamination Solution RNA Isolation Solution 1-Bromo-3-chloropropane Total RNA Isolation Spectrophotometer RNA Concentration Measurement RNA Sample Quality Analysis Agarose Gel Electrophoresis
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