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JoVE Journal Biology
Analyse af SCAP
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Analyse af SCAP

Article DOI: 10.3791/54709-v • 11:27 min • November 8th, 2016
November 8th, 2016 •
1, 1, 1, 1, 1, 1, 1
1Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center and College of Medicine

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Vi beskriver en modificeret fremgangsmåde til membranfraktion isoleret fra humane celler og prøveforberedelse til påvisning af SCAP N -glycosylation og totalt protein ved hjælp af Western blot. Vi yderligere indføre en metode GFP-mærkning for at overvåge SCAP menneskehandel hjælp konfokal mikroskopi. Denne protokol kan bruges i almindelige biologiske laboratorier.

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Cellular Biology SCAP, SREBPs endoplasmatisk reticulum membran protein lipid syntese
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