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Journal / Cancer Research / İlaç Direnci İlgili Roman Splice türevlerini…
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Cancer Research

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JoVE Journal Cancer Research
İlaç Direnci İlgili Roman Splice türevlerini algılamak için RNA sıralamayı kullanarak
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İlaç Direnci İlgili Roman Splice türevlerini algılamak için RNA sıralamayı kullanarak

Article DOI: 10.3791/54714-v • 09:58 min • December 9th, 2016
December 9th, 2016 •
1,2,3, 1, 4, 3, 5, 6, 1,7, *1, *3,8,9
1Department of Pediatric Oncology/Hematology, VU University Medical Center, 2Department of Hematology, VU University Medical Center, 3Department of Medical Oncology, VU University Medical Center, 4Department of Clinical Genetics, VU University Medical Center, 5Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana, Universita’ di Pisa, 6Amsterdam Immunology and Rheumatology Center, VU University Medical Center, 7Princess Máxima Center for Pediatric Oncology, 8Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa, 9Institute of Nanoscience and Nanotechnology, CNR-Nano
* These authors contributed equally

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Burada katı tümörler ve hematolojik malignitelerde ilaç direnci üzerinde anormal yapıştırma etkisini araştıran amaçlayan bir protokol açıklar. Bu hedefe, biz RNA seq ile ebeveyn ve in vitro dirençli modellerin transkriptomik profillerini analiz ve aday genler doğrulamak için bir QRT-PCR tabanlı bir yöntem kurdu.

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Cancer Research Sayı 118 kemoterapi direnç sitotoksisite alternatif birleştirme RNA DİZ transkriptomik
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