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Journal / Cancer Research / 利用RNA测序来检测新剪接变异体在相关耐药…
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Cancer Research

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JoVE Journal Cancer Research
利用RNA测序来检测新剪接变异体在相关耐药
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利用RNA测序来检测新剪接变异体在相关耐药

Article DOI: 10.3791/54714-v • 09:58 min • December 9th, 2016
December 9th, 2016 •
1,2,3, 1, 4, 3, 5, 6, 1,7, *1, *3,8,9
1Department of Pediatric Oncology/Hematology, VU University Medical Center, 2Department of Hematology, VU University Medical Center, 3Department of Medical Oncology, VU University Medical Center, 4Department of Clinical Genetics, VU University Medical Center, 5Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana, Universita’ di Pisa, 6Amsterdam Immunology and Rheumatology Center, VU University Medical Center, 7Princess Máxima Center for Pediatric Oncology, 8Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa, 9Institute of Nanoscience and Nanotechnology, CNR-Nano
* These authors contributed equally

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这里，我们描述旨在调查异常剪接的耐药性在实体瘤和血液恶性肿瘤的影响的协议。为了这个目标，我们通过RNA测序分析，家长和体外抗车型的转录配置文件，并建立了定量RT-PCR为基础的方法来验证候选基因。

Tags

癌症研究，第118，化疗，电阻，细胞毒性，选择性剪接，RNA-SEQ，转录
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