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Journal / Immunology and Infection / Citometria de fluxo de partículas ligadas aos Bet …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Citometria de fluxo de partículas ligadas aos Bet v 1 alérgeno no PM10
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Citometria de fluxo de partículas ligadas aos Bet v 1 alérgeno no PM10

Article DOI: 10.3791/54721-v • 10:42 min • November 19th, 2016
November 19th, 2016 •
1, 1, 1, 1
1Environmental Medicine and Health Effects Assessment, Federal Environment Agency

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Aqui, apresentamos um protocolo para quantificar partículas carregadas de alérgenos por citometria de fluxo. partículas de material particulado ambiental podem agir como portadores de alérgenos adsorvidos. Mostramos aqui que a citometria de fluxo, um método largamente usado para caracterizar os sólidos em suspensão> 0,5 um de diâmetro, pode ser usado para medir estas partículas carregadas com alérgeno.

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Immunology edição 117 Bet v 1 antígeno de pólen de bétula pólen de bétula citometria de fluxo as partículas em suspensão PM10 alérgeno alergia partículas de ar ambiente
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