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Journal / Immunology and Infection / Köpek Nötrofil Ekstrasellüler Trap Release…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Köpek Nötrofil Ekstrasellüler Trap Release Niceleme için Basit Floresan Testi
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Köpek Nötrofil Ekstrasellüler Trap Release Niceleme için Basit Floresan Testi

Article DOI: 10.3791/54726-v • 06:45 min • November 21st, 2016
November 21st, 2016 •
1, 2, 3
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, 2MRC Centre for Inflammation Research, University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University

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Nötrofil dışı tuzaklar (NET) DNA, histon ve nötrofil proteinlerin ağlardır. Doğuştan gelen bağışıklık tepkisinin bir bileşeni olmasına rağmen, ağ otoimmün ve tromboz implike edilmiştir. Bu protokol, köpek nötrofil izolasyon ve bir mikroplaka flüoresans deneyi kullanılarak NET'ler ölçümü için basit bir yöntem tarif eder.

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İmmünoloji Sayı 117 köpek NET nötrofil hücre dışı tuzak floresan mikroplak deneyi köpek
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