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Immunology and Infection

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JoVE Journal Immunology and Infection
A Fluorescence Assay simple pour Quantification de Canine neutrophiles extracellulaires Piège sortie
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A Fluorescence Assay simple pour Quantification de Canine neutrophiles extracellulaires Piège sortie

Article DOI: 10.3791/54726-v • 06:45 min • November 21st, 2016
November 21st, 2016 •
1, 2, 3
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, 2MRC Centre for Inflammation Research, University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University

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pièges extracellulaires neutrophiles (TNE) sont des réseaux d'ADN, les histones et les protéines de neutrophiles. Bien qu'une composante de la réponse immunitaire innée, TNE sont impliqués dans l'auto-immunité et de la thrombose. Ce protocole décrit une méthode simple pour l'isolement des cellules neutrophiles canins et la quantification de TNE en utilisant un dosage de fluorescence de microplaque.

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Immunologie numéro 117 chien NET piège extracellulaire neutrophiles fluorescence dosage de microplaques canin
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