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Journal / Immunology and Infection / Eine einfache Fluoreszenztest zur Quantifizierung …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Eine einfache Fluoreszenztest zur Quantifizierung von Canine Neutrophil Extracellular Einklemmschutz
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Eine einfache Fluoreszenztest zur Quantifizierung von Canine Neutrophil Extracellular Einklemmschutz

Article DOI: 10.3791/54726-v • 06:45 min • November 21st, 2016
November 21st, 2016 •
1, 2, 3
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, 2MRC Centre for Inflammation Research, University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University

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Neutrophile extrazelluläre Fallen (NETs) sind Netzwerke von DNA, Histone und Neutrophilen-Proteine. Obwohl eine Komponente der angeborenen Immunantwort sind NETs in Autoimmun- und Thrombose beteiligt. Dieses Protokoll beschreibt ein einfaches Verfahren zur Hunde- Neutrophilen Isolierung und Quantifizierung von NETs Mikrotiterplatten-Fluoreszenztest verwendet wird.

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Immunologie Heft 117 Hund NET Neutrophilen extrazelluläre Falle Fluoreszenz Mikrotiterplatten-Assay Hunde-
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