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Immunology and Infection

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JoVE Journal Immunology and Infection
En enkel fluorescensanalys för kvantifiering av Canine neutrofila Extracellulär Trap Release
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En enkel fluorescensanalys för kvantifiering av Canine neutrofila Extracellulär Trap Release

Article DOI: 10.3791/54726-v • 06:45 min • November 21st, 2016
November 21st, 2016 •
1, 2, 3
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, 2MRC Centre for Inflammation Research, University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University

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Neutrofila extracellulära fällor (NET) är nätverk av DNA, histoner och neutrofila proteiner. Även om en komponent av den medfödda immunsvar, är nät inblandade i autoimmunitet och trombos. Detta protokoll beskriver en enkel metod för canine neutrofil isolering och kvantifiering av NET med användning av en mikroplattfluorescensanalys.

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Immunologi hund NET neutrofila extracellulärt fälla fluorescens mikroanalys hund
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