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Bestemmelse af den relative celleoverfladen og Total ekspression af rekombinante Ion Channels anvendelse af flowcytometri
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Bestemmelse af den relative celleoverfladen og Total ekspression af rekombinante Ion Channels anvendelse af flowcytometri

Article DOI: 10.3791/54732-v • 11:32 min • September 28th, 2016
September 28th, 2016 •
1, 1, 1, 2, 1
1Département de Physiologie Moléculaire et Intégrative, Montreal Heart Institute Research Centre, 2Département de Microbiologie, Infectiologie, Immunologie, Centre de recherche de l'Hôpital Maisonneuve-Rosemont

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Nedarvede hjertearytmier er ofte forårsaget af mutationer, som ændrer overfladen tilførsel af ét eller flere ionkanaler. Her, tilpasser vi flowcytometri assays til tilvejebringelse af en kvantificering af den relative totale og celleoverfladeprotein ekspression af rekombinante ionkanaler udtrykkes i Tsa-201 celler.

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Cellular Biology Calciumkanaler celleoverfladeprotein ekspression intracellulær proteinekspression rekombinant ekspression flowcytometri
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