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An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

Article DOI: 10.3791/54759-v • 07:23 min • October 10th, 2016
October 10th, 2016 •
1,2, 1, 1,2, 1, 1,2, 1, 1,2
1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 2Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology

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Summary
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A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.

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Optimized Protocol Efficient Radiolabeling Gold Nanoparticles 125I-labeled Azide Prosthetic Group Strain Promoted Copper Free Click Reaction Radiochemistry Field Radioisotope Labeled Nanoparticles Imaging Probes Pad Imaging Studies Spec Imaging Studies Iodine Labeled Particles Azide Prosthetic Group Radiochemical Yield Radiochemical Purity Visual Demonstration Radio Evaluation Steps Radio Iodination Reaction Acetic Acid Precursor Solution Sodium Hydroxide Chloramine T Solution
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