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JoVE Journal
Biology

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三维超F-微丝高分辨率显微镜通过干涉光敏定位显微镜(iPALM)
 
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三维超F-微丝高分辨率显微镜通过干涉光敏定位显微镜(iPALM)

Article DOI: 10.3791/54774-v 11:57 min December 1st, 2016
December 1st, 2016

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Summary

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Click here for the English version.

我们提出了一个协议,用于干涉式光活化的定位显微镜(iPALM),3维单分子定位超分辨率显微镜方法的应用,以便在贴壁哺乳动物细胞的肌动蛋白细胞骨架的成像。这种方法允许的纳米结构特征,否则将通过常规的衍射限制的光学显微镜仍未解决基于光的可视化。

Tags

生物物理学,第118,超高分辨率显微镜,PALM,iPALM,荧光,F-肌动蛋白,单分子定位,光开关荧光团,干涉
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