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Journal / Developmental Biology / Visualizing the Interrenal Steroidogenic Tissue…
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Developmental Biology

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JoVE Journal Developmental Biology
Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
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Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Article DOI: 10.3791/54820-v • 07:19 min • December 21st, 2016
December 21st, 2016 •
1, 1, 1, 1
1Department of Life Science, Tunghai University

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The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-β-hydroxysteroid dehydrogenase (Δ5-4 isomerase; 3β-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish.

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Interrenal Steroidogenic Cells Zebrafish Enzymatic Activity Assay Staining Procedure Genetic Or Chemical Screens Fluorescent Reporter Lines Kidney Blood Vessels Vibratome Sectioning Immunohistochemistry Confocal Microscopy Beta-Hsd Activity Staining Developing Zebrafish Embryos PTU Dechorionated Embryos PFA PBST
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