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JoVE Journal Developmental Biology
Het visualiseren van de Interrenal steroïdogene Tissue en haar Vasculaire Microenvironment in zebravis
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Het visualiseren van de Interrenal steroïdogene Tissue en haar Vasculaire Microenvironment in zebravis

Article DOI: 10.3791/54820-v • 07:19 min • December 21st, 2016
December 21st, 2016 •
1, 1, 1, 1
1Department of Life Science, Tunghai University

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De interrenal klier in de zebravis is de teleostean tegenhanger van de zoogdieren bijnier. Dit protocol wordt beschreven hoe de 3-β-hydroxysteroïdedehydrogenase (Δ 5-4 isomerase, 3β-Hsd) verricht enzymatische activiteit test, die gedifferentieerde steroïdogene cellen in zebravisembryo detecteert.

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Developmental Biology 3-β-hydroxysteroïddehydrogenase zebravis steroïdogene adrenocorticale interrenal nieren bloedvaten micro vibratome snijden immunohistochemie
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