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Journal / Developmental Biology / Visualizzare il Tissue interrenale Steroidogenic…
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JoVE Journal Developmental Biology
Visualizzare il Tissue interrenale Steroidogenic e la sua Vascolare Microenvironment in Zebrafish
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Visualizzare il Tissue interrenale Steroidogenic e la sua Vascolare Microenvironment in Zebrafish

Article DOI: 10.3791/54820-v • 07:19 min • December 21st, 2016
December 21st, 2016 •
1, 1, 1, 1
1Department of Life Science, Tunghai University

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La ghiandola interrenale in zebrafish è la controparte teleostei della ghiandola surrenale mammiferi. Questo protocollo introduce come eseguire la deidrogenasi 3-β-idrossisteroide (Δ 5-4 isomerasi; 3β-HSD) saggio di attività enzimatica, che rileva le cellule differenziate steroidogeniche nel zebrafish in via di sviluppo.

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Biologia dello Sviluppo Numero 118 deidrogenasi 3-β-idrossisteroide zebrafish steroidea surrenalica interrenale reni vasi sanguigni microambiente vibratome sezionamento immunoistochimica
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