Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Development and Identification of a Novel…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice
Journal (Immunology and Infection)
Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
Journal (Immunology and Infection)
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
 

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Article DOI: 10.3791/54826-v • 10:05 min • January 25th, 2017
January 25th, 2017 •
1, 1, 1, 1, 1
1The Lloyd Rigler Sleep Apnea Research Laboratory, Unit of Anatomy and Cell Biology, The Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Insitute of Technology

Chapters

Summary
Automatic Translation

We describe here a method for obtaining and identifying a newly characterized subpopulation of neutrophil-derived giant phagocytes. These cells develop in culture from fresh human blood neutrophils, and are characterized by phagocytosis, autophagy, immensely large size, and extended lifespan. This method is essential to further investigate this unique neutrophil-derived subpopulation.

Tags

Development Identification Novel Subpopulation Human Neutrophil-derived Giant Phagocytes In Vitro Inflammatory Anti-inflammatory Immune Responses Long-term Culture Conditions Significance Functions Activation Plasticity Research Assistant Post Doc Sterile Scalp Vein Set Venous Blood Biosafety Laminar Flow Hood Dilute Aliquots Whole Blood Sample ION-free PBS Heat Inactivated FCS Polysucrose 1119 Polysucrose 1077 Polypropylene Conical Centrifuge Tube
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter