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Journal / Genetics / Measurement of Differentially Methylated INS DNA…
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JoVE Journal Genetics
Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death
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Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

Article DOI: 10.3791/54838-v • 10:34 min • December 21st, 2016
December 21st, 2016 •
1, 1, 2, 1,3,4
1Department of Pediatrics, IU Center for Diabetes and Metabolic Disease, Indiana University School of Medicine, 2Department of Pediartics, Omaha Children's Hospital and Medical Center, University of Nebraska Medical Center, 3Departments of Biochemistry and Molecular Biology, Medicine, and Cellular and Integrative Physiology, IU Center for Diabetes and Metabolic Disease, Indiana University School of Medicine, 4Indiana Biosciences Research Institute

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Summary
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Islet β cell death precedes development of type 1 diabetes, and detecting this process may allow for early therapeutic intervention. Here, we provide a detailed description of how to measure differentially methylated INS DNA species in human serum as a biomarker of β cell death.

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Differentially Methylated INS DNA Human Serum Samples Biomarker Islet Beta Cell Death Diabetes Digital PCR Serum Collection Lysis Buffer Poly(A) Protease Incubation Centrifuge DNA Precipitation Ethanol Spin Columns
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