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Journal / Biochemistry / 在线体积排阻和离子交换色谱法SAXS光束线…
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Biochemistry

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JoVE Journal Biochemistry
在线体积排阻和离子交换色谱法SAXS光束线
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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在线体积排阻和离子交换色谱法SAXS光束线

Article DOI: 10.3791/54861-v • 11:09 min • January 5th, 2017
January 5th, 2017 •
1, 2,3, 4
1Structural Biology Group, European Synchrotron Radiation Facility, 2European Molecular Biology Laboratory, 3School of Chemical and Physical Sciences, Keele University, 4Groupe de Microscopie Electronique et Méthodes, Institut de Biologie Structurale

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通过小角X射线散射（SAXS）的蛋白的溶液结构的确定需要单分散样品。在这里，我们提出了两种可能性，以确保样品制备和数据采集之间的最小延迟：在线尺寸排阻色谱（SEC）和在线离子交换层析（IEC）。

Tags

生物化学，第119，生物小角X射线散射（BioSAXS），在线尺寸排阻色谱法（SEC），在线离子交换色谱（IEC），背景减除，D5，牛痘
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