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JoVE Journal Bioengineering
Design og implementering af en automatiseret lysende, Dyrkning, og Sampling System for Mikrobiel optogenetic Applications
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Design og implementering af en automatiseret lysende, Dyrkning, og Sampling System for Mikrobiel optogenetic Applications

Article DOI: 10.3791/54894-v • 11:13 min • February 19th, 2017
February 19th, 2017 •
1, 1
1Department of Biomedical Engineering, University of Wisconsin-Madison

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Vi designede en kontinuerlig dyrkning apparat til brug med optogenetic systemer til at belyse kulturer af mikrober og regelmæssigt billedceller i effluenten med et inverteret mikroskop. Dyrkning, prøveudtagning, billedbehandling og billedanalyse er fuldt automatiseret, så dynamiske reaktioner på belysning kan måles over flere dage.

Tags

Bioengineering optogenetics mikrobiologi syntetisk biologi kemostat, Knopskydende gær kontrol identifikationssystem proteinekspression
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