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Journal / Immunology and Infection / Optimering af en kvantitativ…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Optimering af en kvantitativ Micro-neutraliseringsanalyse
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Optimering af en kvantitativ Micro-neutraliseringsanalyse

Article DOI: 10.3791/54897-v • 10:09 min • December 14th, 2016
December 14th, 2016 •
1, 1, 1
1Mill Hill Laboratory, The Francis Crick Institute

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Denne undersøgelse beskriver en imaging-baserede mikro-neutraliseringsanalyse at analysere de antigene relationer mellem vira. Protokollen anvender en flatbed scanner og har fire trin, herunder titrering, titrering kvantificering, neutralisering, og neutralisering kvantificering. Assayet fungerer godt med nuværende cirkulerende influenza A (H1N1) pdm09, A (H3N2), og B-virus.

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Infektion influenza mikro-neutralisering hæmagglutinationsinhiberings antigenicitet flatbed scanner high-throughput billedbehandling virus-inficerede celle kvantificering plaque assay
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