Laser-Induced Chronic Ocular Hypertension Model on SD Rats

Published 12/04/2007
1 Comment
  CITE THIS  SHARE 
Biology

Your institution must subscribe to JoVE's Biology section to access this content.

Fill out the form below to receive a free trial or learn more about access:

Welcome!

Enter your email below to get your free 10 minute trial to JoVE!





By clicking "Submit", you agree to our policies.

 

Summary

Glaucoma is one of the major causes of blindness in the world. Elevated intraocular pressure is a major risk factor. Laser photocoagulation induced ocular hypertension is one of the well established animal models. This video demonstrates how to induce ocular hypertension by Argon laser photocoagulation in rat.

Cite this Article

Copy Citation

Chiu, K., Chang, R., So, K. Laser-Induced Chronic Ocular Hypertension Model on SD Rats. J. Vis. Exp. (10), e549, doi:10.3791/549 (2007).

Abstract

Glaucoma is one of the major causes of blindness in the world. Elevated intraocular pressure is a major risk factor. Laser photocoagulation induced ocular hypertension is one of the well established animal models. This video demonstrates how to induce ocular hypertension by Argon laser photocoagulation in rat.

Protocol

Check and prepare the equipments

  1. Anaesthetize the rat by intra-peritoneal injection of ketamine (80mg/kg) and xylazine (8mg/kg) (volume ratio at 2:1).
  2. Apply one drop of 0.5% alcaine to the rat eyes as topical anesthetics before laser photocoagulation.
  3. Position the rat and expose the target veins with a curved forceps. Use the footstep to start laser photocoagulation. Apply bout 60 laser spots around the limbal vein (except the nasal area) and 15-20 laser spots on each episcleral aqueous humor drainage vein.

Laser photocoagulation

  1. Anaesthetize the rat by intra-peritoneal injection of ketamine (80mg/kg) and xylazine (8mg/kg) (volume ratio at 2:1).
  2. Apply one drop of 0.5% alcaine to the rat eyes as topical anesthetics before laser photocoagulation.
  3. Position the rat and expose the target veins with a curved forceps. Use the footstep to start laser photocoagulation. Apply bout 60 laser spots around the limbal vein (except the nasal area) and 15-20 laser spots on each episcleral aqueous humor drainage vein.
  4. After each laser treatment, apply ophthalmic Tobrex ointment on the rat eye to prevent infection.
  5. Switch off the laser system by turning the key anti-clockwise to point "0".
  6. Switch off slit lamp by turning the switch to point "0".

Subscription Required. Please recommend JoVE to your librarian.

Discussion

After two laser treatments with 7 days apart, we can elevate the IOP of laser photocoagulated eye by 50% compare with control eye. The elevated IOP can sustain for at least for 3 month after first laser treatment. This rat model of elevated intraocular pressure provide valuable opportunities to study the mechanisms of pressure-induced retinal ganglion cell loss and optic nerve damage that mimic the pathological change in human glaucoma.

Subscription Required. Please recommend JoVE to your librarian.

Disclosures

The authors have nothing to disclose.

Materials

Name Type Company Catalog Number Comments
ULTIMA® 2000SE Argon Laser Tool Coherent Inc.
Protective goggles Other Coherent Inc.

DOWNLOAD MATERIALS LIST

References

  1. Chan, H. -C., Chang, R. C. C., Koon-Ching Ip, A., Chiu, K., Yuen, W. -H., Zee, S. -Y., So, K. -F. Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma. Experimental Neurology. 203, 269-273 (2007).
  2. Ji, J. -Z., Elyaman, W., Yip, H. K., Lee, V. W. H., Yick, L. -W., Hugon, J., So, K. -F. CNTF promotes survival of retinal ganglion cells after induction of ocular hypertension in rats: the possible involvement of STAT3 pathway. European Journal of Neuroscience. 19, 265-272 (2004).
  3. Li, R. S., Chen, B. -Y., Tay, D. K., Chan, H. H. L., Pu, M. -L., So, K. -F. Melanopsin-Expressing Retinal Ganglion Cells Are More Injury-Resistant in a Chronic Ocular Hypertension Model. Investigative Ophthalmology & Visual Science. 47, 2951-2958 (2006).
  4. Schori, H., Kipnis, J., Yoles, E., WoldeMussie, E., Ruiz, G., Wheeler, L. A., Schwartz, M. Vaccination for protection of retinal ganglion cells against death from glutamate cytotoxicity and ocular hypertension: Implications for glaucoma. PNAS. 98, 3398-3403 (2001).
  5. WoldeMussie, E., Ruiz, G., Wijono, M., Wheeler, L. A. Neuro-protection of retinal ganglion cells by brimonidine in rats with laser-induced chronic ocular hypertension. Investigative Ophthalmology & Visual Science. 42, 2849-2855 (2001).

Comments

1 Comment

  1. Dear Chiu, What about the size of the laser spot? and for the second laser,how to identify the veins? as  the veins  would be  more difficult to identify  after the first laser . Thanks a lot! Best egards, Huang Chukai

    Reply
    Posted by: Anonymous
    January 13, 2009 - 9:40 AM

Post a Question / Comment / Request

You must be signed in to post a comment. Please or create an account.

Video Stats