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JoVE Journal
Developmental Biology

This content is Open Access.

诱导多能干细胞的快速神经元分化有关微电极阵列测量网络活动
 
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诱导多能干细胞的快速神经元分化有关微电极阵列测量网络活动

Article DOI: 10.3791/54900-v 09:20 min January 8th, 2017
January 8th, 2017

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Summary

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Click here for the English version.

我们修改和实施描述人类诱导多能干细胞(iPS细胞)的快速,可重复,高效分化成兴奋皮层神经元12先前公布的协议。具体地讲,我们的修改允许对神经元细胞密度和使用的控制上的微电极阵列以测量在网络级电生理特性。

Tags

发育生物学,第119,诱导多能干细胞,神经元分化,微电极阵列,慢病毒转导,星形胶质细胞隔离,神经网络
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