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Journal / Developmental Biology / Hurtig neuronal differentiering af induceret…
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Developmental Biology

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JoVE Journal Developmental Biology
Hurtig neuronal differentiering af induceret pluripotente stamceller til måling Network Aktivitet på Micro-elektrode Arrays
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Hurtig neuronal differentiering af induceret pluripotente stamceller til måling Network Aktivitet på Micro-elektrode Arrays

Article DOI: 10.3791/54900-v • 09:20 min • January 8th, 2017
January 8th, 2017 •
*1,2, *3, 2,3, 3, 1,2, 1,2, 1,2, 2,3,4, 1,2,3
1Department of Cognitive Neurosciences, Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour, Radboud University, 3Department of Human Genetics, Radboudumc, 4Department of Molecular Developmental Biology, Radboud University
* These authors contributed equally

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Vi ændre og gennemføre en tidligere offentliggjort protokol beskriver hurtig, reproducerbar og effektiv differentiering af humane inducerede pluripotente stamceller (hiPSCs) i excitatoriske kortikale neuroner 12. Specifikt vores modifikation giver mulighed for kontrol af neuronal celletæthed og brug på mikroelektrode-arrays til at måle elektrofysiologiske egenskaber på netværksniveau.

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Developmental Biology inducerede pluripotente stamceller neuronal differentiering mikro-elektrodesæt lentiviral transduktion astrocyt isolation neuronal netværk
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