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Journal / Bioengineering / 2D- og 3D-matriser for å studere lineær…
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JoVE Journal Bioengineering
2D- og 3D-matriser for å studere lineær invadosomdannelse og aktivitet
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2D- og 3D-matriser for å studere lineær invadosomdannelse og aktivitet

Article DOI: 10.3791/54911-v • 12:26 min • June 2nd, 2017
June 2nd, 2017 •
*1,2, *1, 1,3,4, 2, 2, 1, 1
1INSERM U1053, 2Icahn School of Medicine at Mount Sinai, Tisch Cancer Institute, 3Faculté de Médecine, Casablanca, Université Mohammed 6 des Sciences de la Santé (UM6SS), 4Laboratoire National de Référence
* These authors contributed equally

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Denne protokollen beskriver hvordan du skal lage en 2D blandet matrise, bestående av gelatin og kollagen I, og en 3D kollagen I-plugg for å studere lineære invadosomer. Disse protokollene tillater studier av lineær invadosomdannelse, matriksdegradasjonsaktivitet og invasjonsegenskapene til primære celler og kreftcellelinjer.

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Bioengineering utgave 124 Lineær invadosom ekstracellulær matrise nedbrytningsaktivitet kollagen I 3D invasjon
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