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JoVE Journal Developmental Biology
Co-Lokalisierung von Zell Lineage Marker und die Tomate Signal
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Co-Lokalisierung von Zell Lineage Marker und die Tomate Signal

Article DOI: 10.3791/54982-v • 10:56 min • December 28th, 2016
December 28th, 2016 •
1, 1, 1, 1
1Department of Biomedical Sciences, Texas A&M University College of Dentistry

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Wir entwickelten zwei Sätze von Kombinationen in Rosa26 tdTomato Tracing (ubiquitär in allen Zellen exprimiert) / Cre (insbesondere in Chondrozyten exprimiert) Mäuse: eine mit 2.3Col1a1-GFP (spezifisch für Osteoblasten) und eine mit Immunofluoreszenz (spezifisch für Knochenzellen). Die Daten zeigen die direkte Umwandlung von Chondrozyten in Knochenzellen.

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Entwicklungsbiologie Heft 118 Zelllinie Tracing Zelle transdifferentiation Immunofluoreszenz Chondrozyten Osteoblasten Osteozyten
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