Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / In vitro Differenzierung von humanen CD4 + FOXP3…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
In vitro Differenzierung von humanen CD4 + FOXP3 + Induced regulatorischer T - Zellen (iTregs) von Naïve CD4 + T - Zellen unter Verwendung eines TGF-β-enthaltende Protokoll
Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Journal (Immunology and Infection)
Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples
Journal (Immunology and Infection)
Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples
 
Click here for the English version

In vitro Differenzierung von humanen CD4 + FOXP3 + Induced regulatorischer T - Zellen (iTregs) von Naïve CD4 + T - Zellen unter Verwendung eines TGF-β-enthaltende Protokoll

Article DOI: 10.3791/55015-v • 08:20 min • December 30th, 2016
December 30th, 2016 •
1, *1, *1, 1
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Dieses Protokoll beschreibt die reproduzierbare Erzeugung und Phänotypisierung von humanen regulatorischen T - Zellen induziert (iTregs) von naiven CD4 + T - Zellen in vitro. Verschiedene Protokolle für FOXP3 Induktions ermöglichen die Untersuchung spezifischer iTreg Phänotypen mit jeweiligen Protokollen erhalten.

Tags

Immunologie Heft 118 regulatorische T-Zellen Treg CD4 + T-Zellen magnetische Zellisolierung, Cytokinen FOXP3 TGF-β IL-2 intrazelluläre Durchflusszytometrie qRT-PCR
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter