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Journal / Immunology and Infection / In vitro Differensiering av Menneskelig CD4 +…
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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro Differensiering av Menneskelig CD4 + foxp3 + Induced regulatoriske T-celler (iTregs) fra naive CD4 + T-celler under anvendelse av et TGF-β holdige Protocol
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In vitro Differensiering av Menneskelig CD4 + foxp3 + Induced regulatoriske T-celler (iTregs) fra naive CD4 + T-celler under anvendelse av et TGF-β holdige Protocol

Article DOI: 10.3791/55015-v • 08:20 min • December 30th, 2016
December 30th, 2016 •
1, *1, *1, 1
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
* These authors contributed equally

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Denne protokollen beskriver reproduserbar generering og fenotyping av menneskeskapte regulatoriske T-celler (iTregs) fra naive CD4 + T-celler in vitro. Forskjellige protokoller for foxp3 induksjons tillater studiet av spesifikke fenotyper iTreg oppnådd med respektive protokoller.

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Immunologi regulatoriske T-celler treg CD4 + T-celler magnetisk celle isolasjon, cytokiner foxp3 TGF-β IL-2 intracellulær Strømningscytometri QRT-PCR
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