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Journal / Biochemistry / SorLA og CLC: CLF-1-afhængig Nedregulering af…
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Biochemistry

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JoVE Journal Biochemistry
SorLA og CLC
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SorLA og CLC: CLF-1-afhængig Nedregulering af CNTFRa som demonstreret ved Western blotting, Inhibering af lysosomale enzymer, og Immunocytokemi

Article DOI: 10.3791/55019-v • 10:16 min • January 6th, 2017
January 6th, 2017 •
1, 1
1Department of Biomedicine, Aarhus University

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Den nuværende protokol beskriver, hvordan Western blotting og immuncytokemi kombineret med hæmning af lysosomale enzymer kan bruges til at demonstrere nedregulering af ciliarneurotrofiske Factor receptor-α (CNTFRa), der transporteres af samspillet mellem Cytokine-lignende faktor-1 (CLF-1 ) og den endocytiske receptor sorLA.

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Biokemi CLC: CLF-1 NNT-1 BSF-3 CRLF1 CNTFRa sorLA Vps10p STAT3
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