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JoVE Journal
Biochemistry

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SorLA en CLC
 
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SorLA en CLC: CLF-1-afhankelijke negatieve regulatie van CNTFRa zoals aangetoond door Western blotting, Remming van lysosomale enzymen en immunocytochemie

Article DOI: 10.3791/55019-v 10:16 min January 6th, 2017
January 6th, 2017

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Summary

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Click here for the English version.

Dit protocol beschrijft hoe Western blotting en immunocytochemie gecombineerd met remming van lysosomale enzymen kunnen worden gebruikt om de neerwaartse regulatie van ciliaire neurotrofe factor receptor-α (CNTFRa), die wordt overgebracht door de interactie tussen cytokine-achtige factor-1 (CLF-1 tonen ) en de endocytosereceptor sorLA.

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Biochemie CLC: CLF-1 NNT-1 BSF-3 CRLF1 CNTFRa sorLA Vps10p STAT3
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