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Journal / Developmental Biology / Insamling av Serum och Feeder fria Mouse…
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JoVE Journal Developmental Biology
Insamling av Serum och Feeder fria Mouse embryonala stamceller konditionerade medium för ett cellfritt Approach
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Insamling av Serum och Feeder fria Mouse embryonala stamceller konditionerade medium för ett cellfritt Approach

Article DOI: 10.3791/55035-v • 09:32 min • January 8th, 2017
January 8th, 2017 •
1, 2,3, 1
1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, 2Physiology and Experimental Medicine Program, The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

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Detta protokoll ger en metod för insamling av mus embryonala stamceller (Mesc) -konditionerat medium (Mesc-CM) härstammar från serum (fetalt bovint serum, FBS) - och matar (mus embryonala fibroblaster, MEF) -fria förutsättningar för en cell -fri tillvägagångssätt. Det kan vara tillämplig för behandling av åldrande och åldersassocierade sjukdomar.

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Developmental Biology cellulärt åldrande Konditionerat medium (CM) Embryonala stamceller (ESCS) Cellfri tillvägagångssätt Serumfritt Feeder fritt
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