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Journal / Developmental Biology / Innsamling av serum-og mater-fri Mouse Embryonic…
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Developmental Biology

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JoVE Journal Developmental Biology
Innsamling av serum-og mater-fri Mouse Embryonic Stem Cell kondisjonerte Medium for en Cell-fri tilnærming
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Innsamling av serum-og mater-fri Mouse Embryonic Stem Cell kondisjonerte Medium for en Cell-fri tilnærming

Article DOI: 10.3791/55035-v • 09:32 min • January 8th, 2017
January 8th, 2017 •
1, 2,3, 1
1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, 2Physiology and Experimental Medicine Program, The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

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Denne protokollen gir en fremgangsmåte for innsamling av muse-embryonale stamceller (Mesc) -conditioned medium (Mesc-CM) avledet fra serum (føtalt bovint serum, FBS) - og mater (mus embryonale fibroblaster, MEFs) -fri forhold for en celle -gratis tilnærming. Det kan være aktuelt for behandling av aldring og aldring-assosierte sykdommer.

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Developmental Biology Cellular senescence Egnet media (CM) embryonale stamceller (ESCs) Cell-fri tilnærming Serum-free mater-fri
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