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Immunology and Infection

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Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Article DOI: 10.3791/55087-v • 10:39 min • March 10th, 2017
March 10th, 2017 •
*1,2, *3, 4, 5, 6,7, 6,7, 1
1Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, 2Department of Basic Biomedical Science, Universidad Europea de Madrid, 3Human Biology Program, School of Integrative and Global Majors, University of Tsukuba, 4Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa, Instituto de Salud Carlos III, 5Division of Microbiology, Department of Medicine, School of Medicine, Universidad Complutense, 6Biology of Gram-Positive Pathogens, Department of Microbiology, Institut Pasteur, Paris, France, 7ERL3526, CNRS, Paris, France
* These authors contributed equally

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Summary
Automatic Translation

We describe here three different protocols for the in vitro investigation of conjugation, transduction, and natural transformation in Staphylococcus aureus.

Tags

Methodology Study Horizontal Gene Transfer Staphylococcus Aureus Conjugation Transduction Natural Transformation Antibiotic Resistant Genes Human Pathogen Experiment Overnight Culture Tryptic Soy Broth Chloramphenicol Optical Density Phosphate Buffered Saline PBS Filter Membrane Sheep Blood Agar Plate
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