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Journal / Immunology and Infection / Imaging den neutrofila fagosomen och Cytoplasma…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Imaging den neutrofila fagosomen och Cytoplasma hjälp av en Propportionell pH-indikator
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Imaging den neutrofila fagosomen och Cytoplasma hjälp av en Propportionell pH-indikator

Article DOI: 10.3791/55107-v • 12:42 min • April 5th, 2017
April 5th, 2017 •
1, 1, 1, 2, 1
1Centre for Molecular Medicine, Division of Medicine, University College London, 2Cell and Developmental Biology, University College London

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Detta manuskript beskriver en enkel metod för att mäta phagosomal pH och område samt den cytoplasmatiska pH hos människa och mus neutrofiler med användning av ratiometrisk indikatorn seminaphthorhodafluor (Snarf) -1, eller S-1. Detta uppnås med hjälp av live-cell konfokal fluorescensmikroskopi och bildanalys.

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Immunologi fagocytos neutrofil phagosomal pH ratiometrisk avbildning fluorescensmikroskopi medfödd immunitet
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