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Immunology and Infection

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JoVE Journal Immunology and Infection
Genome-wide analys av HDAC-hämmare-medierad modulering av mikroRNA och mRNA i B celler förmås att genomgå klass-switch DNA-rekombination och Plasma celldifferentiering
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Genome-wide analys av HDAC-hämmare-medierad modulering av mikroRNA och mRNA i B celler förmås att genomgå klass-switch DNA-rekombination och Plasma celldifferentiering

Article DOI: 10.3791/55135-v • 11:06 min • September 20th, 2017
September 20th, 2017 •
1, 1,2, 1,3, 1,3, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine, Cental South University, 3Greehey Children’s Cancer Research Institute, University of Texas Long School of Medicine at San Antonio

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Epigenetiska faktorer kan interagera med genetiska program att modulera genuttryck och reglera B cellsfunktion. Genom att kombinera in vitro B-cell stimulering, qRT-PCR, och hög genomströmning mikroRNA-sekvens och mRNA-sekvens tillvägagångssätt, kan vi analysera de epigenetiska moduleringen av miRNA och gen uttryck i B-celler.

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Immunologi problemet 127 B-cell klass-switch DNA-rekombination flödescytometri Histon histondeacetylas (HDAC)-hämmare (HDI) mRNA mikroRNA mRNA-seq miRNA-seq plasma celldifferentiering
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