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Journal / Immunology and Infection / Legionella pneumophila der äußeren Membran -…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Legionella pneumophila der äußeren Membran - Vesikeln
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Legionella pneumophila der äußeren Membran - Vesikeln: Isolierung und Analyse ihrer Pro-inflammatorische Potential auf Makrophagen

Article DOI: 10.3791/55146-v • 08:35 min • February 22nd, 2017
February 22nd, 2017 •
1, 1, 1, 1, 1, 1,2,3
1Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg

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Hier beschreiben wir die Reinigung von Legionella pneumophila (L. pneumophila) , äußere Membranvesikel (OMVs) aus Flüssigkulturen. Diese gereinigten Vesikel werden dann für die Behandlung von Makrophagen verwendet, ihre pro-inflammatorischen Potentials zu analysieren.

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Die Infektion Ausgabe 120, Äußere Membranvesikel Makrophagen Aufreinigung differentielle Ultrazentrifugation pro-inflammatorische Antwort bakterielle Replikations
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