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JoVE Journal Developmental Biology
Effektiv Isolering af Functional Islets fra Neonatal Mouse Pancreas
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Effektiv Isolering af Functional Islets fra Neonatal Mouse Pancreas

Article DOI: 10.3791/55160-v • 07:04 min • January 6th, 2017
January 6th, 2017 •
1, 1
1Department of Cell and Development Biology, Vanderbilt University Medical School

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Vi beskriver en protokol heri til isolering intakte øer fra neonatale mus. Pancreas blev delvist fordøjet med collagenase, efterfulgt af vask og hånd plukning. kan opnås 80 ø-klynger pr pancreas fra nyfødte mus, som er egnede til flere ø-undersøgelser - 20.

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Developmental Biology transplantation collagenase diabetes modning Islets insulin beta-celler sekretion neonatal hånd plukning
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