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JoVE Journal
Biology

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线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在
 
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线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在

Article DOI: 10.3791/55166-v 08:43 min January 24th, 2017
January 24th, 2017

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Summary

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Click here for the English version.

线粒体可以利用在其内的膜(Δ 膜电势 )的电化学势螯合钙(Ca 2+),允许它们塑造细胞内的Ca 2+的细胞内信号传导。我们描述了使用荧光染料和共聚焦显微镜同时测量线粒体的Ca 2+摄取和ΔΨ 微米活细胞的方法。

Tags

细胞生物学,第119,线粒体,膜电位,钙,荧光染色,活细胞,共聚焦显微镜
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